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RT-PCR analysis of apoptosis-related genes in normal human osteoblasts infected with S. aureus at an MOI of 250:1 (S. aureus organisms to osteoblasts). PCR amplification was performed as recommended by BioSource International. Gene expression was monitored at 6 h following the addition of viable bacteria. Bcl-xL, B-cell lymphoma gene × Long; Bcl-2, B-cell lymphoma 2; Bax, Bcl-2-associated × protein; Fas, CD95; Fas-L, Fas ligand.
