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RT-RCR analysis of TRAIL and TRAIL receptor (TRAIL/R) expression in normal mouse osteoblasts infected with Salmonella serovar Dublin or UV-killed Salmonella serovar Dublin at different MOIs (10:1, 3:1, or 1:1 [Salmonella serovar Dublin organisms to osteoblasts]). PCR amplification of G3PDH was performed to ensure equal input of RNA and similar efficiencies of RT. Gene expression was monitored at 0.5 and 6 h following the addition of viable or UV-killed bacteria.
