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RT-RCR analysis of TRAIL, TRAIL R1, and TRAIL R2 expression in normal human osteoblasts infected with S. aureus or Salmonella serovar Dublin at different MOIs (25:1, 75:1, or 250:1 [S. aureus organisms to osteoblasts]; 1:1, 3:1, or 10:1 [Salmonella serovar Dublin organisms to osteoblasts]). PCR amplification of G3PDH was performed to ensure equal input of RNA and similar efficiencies of RT. Gene expression was monitored at 6 h following the addition of viable bacteria.
