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Western immunoblot analysis of cell-associated TRAIL expression by normal human osteoblasts infected with S. aureus or UV-killed S. aureus at different MOIs (25:1, 75:1, or 250:1 [S. aureus organisms to osteoblasts]). TRAIL expression was monitored at 45 min following the addition of viable or UV-killed bacteria and at 6, 12, and 24 h following infection with viable S. aureus cells. Two micrograms of total protein was loaded for each lane.
