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. 2001 Mar;69(3):1581–1586. doi: 10.1128/IAI.69.3.1581-1586.2001

FIG. 9.

FIG. 9

Western immunoblot analysis of TRAIL secretion by normal human osteoblasts infected with Salmonella serovar Dublin or UV-killed Salmonella serovar Dublin at different MOIs. Lanes, from left to right, contained protein reactive with anti-TRAIL antibody in culture supernatants 45 min following infection with Salmonella serovar Dublin at an MOI of 0, 1:1, 3:1, or 10:1; protein reactive with anti-TRAIL antibody in culture supernatants 45 min following the addition of UV-killed Salmonella serovar Dublin at an MOI of 3:1 or 10:1; and protein reactive with anti-TRAIL antibody in culture supernatants at 6, 12, and 24 h following infection with Salmonella serovar Dublin at an MOI of 10:1. Ten micrograms of total protein from serum-containing medium was loaded for each lane.