Table 2.
Matrix effects and extraction recovery rate of surface plasmon resonance (SPR) and ultra-performance liquid chromatography-ultraviolet (UPLC-UV) analyses (n = 3).
| Methods | Nominal concentration | Mean response (RU)/Mean peak area a |
Matrix effect (%) | Extraction recovery rate (%) | ||
|---|---|---|---|---|---|---|
| A1 (A2) | B1 (B2) | C1 (C2) | ||||
| SPR | 0.3 ng/mL | 2.33 | 1.57 | 1.59 | 68 | 99 |
| 3.0 ng/mL | 13.78 | 11.76 | 14.83 | 108 | 79 | |
| 30.0 ng/mL | 47.36 | 44.49 | 44.64 | 94 | 100 | |
| UPLC-UV | 0.2 μg/mL | 1.08 | 0.76 | 0.83 | 76 | 92 |
| 1.0 μg/mL | 4.19 | 3.55 | 3.92 | 94 | 90 | |
| 5.0 μg/mL | 22.24 | 17.91 | 20.57 | 92 | 87 | |
a
Groups A1, B1, and C1 are analyzed by SPR method and groups A2, B2, and C2 are analyzed by UPLC-UV method. Groups A1 and A2 were the mean response and mean peak area of the standard solution without serum, respectively; groups B1 and B2 were the mean response and mean peak area of the blank serum sample with standard solution added before protein precipitation, respectively; and groups C1 and C2 were the mean response and mean peak area of the blank serum sample with standard solution added after protein precipitation, respectively.