FIG. 3.

MBP-A binds deglycosylated CHO CD14H. CD14H (4 μg) produced in CHO cells (A) or CHO CD14H treated with N-glycosidase F, neuraminidase, and O-glycosidase (deglycosylated CHO CD14) (B) was electrophoresed and visualized by Coomassie brilliant blue staining. The proteins were also transferred onto PVDF membranes and incubated with MBP-A (2 μg/ml) or BSA at room temperature for 3 h, and those binding to the membranes were detected by anti-MBP-A antibody (Ab) (ligand binding) as described in Materials and Methods.