FIG. 1.

Seroconservation of proteins Sp101 and Sp130 among pneumococcal strains. Whole-cell lysates were prepared from strains with serotypes representative of those contained in current 23-valent carbohydrate vaccines (serotypes 1, 2, 3, 4, 5, 6B, 7F, 8, 9N, 9V, 10A, 11A, 12F, 14, 15B, 17F, 18C, 19A, 19F, 20, 22F, 23F, and 33F) or from clinical isolates N4 (serotype 4 strain; source of genomic DNA used in sequencing), SJ2 (serotype 6B), and WU2 and A66 (serotype 3). Proteins in lysates were separated by electrophoresis and immunoblots were probed with rabbit antisera raised against either recombinant Sp101 or Sp130.