FIG. 3.

Immune recognition of pneumococcal vaccine proteins by convalescent-phase sera from patients diagnosed with pneumococcal infections. Recombinant pneumococcal proteins were resolved by polyacrylamide gel electrophoresis and transferred to nitrocellulose membranes. Membranes were probed with convalescent-phase sera obtained from patients recovering from bacteremic pneumococcal pneumonia. Bound antibody was detected with an enzyme-conjugated secondary antibody specific for human immunoglobulin.