. 2001 Mar;69(3):1593–1598. doi: 10.1128/IAI.69.3.1593-1598.2001

TABLE 2.

Active immunization of C3H/HeJ mice with recombinant pneumococcal proteins in Freund's adjuvant protects mice from a lethal challenge with a serotype 6B strain of pneumococci

Antigen	Motif	Accession no.	Subcellular location^a	Database similarity^b (accession no.)	Expt	No. of survivors/total no.
Antigen	Motif	Accession no.	Subcellular location^a	Database similarity^b (accession no.)	Expt	Active immunization^c	Sham immunization
Sp36	SPase II	AF291695	CW/M	Unknown protein from Streptococcus agalactiae (AAD13797)	1	9/10	0/10
				Unknown protein from Streptococcus agalactiae (AAD13797)	2	10/10	0/10
Sp46	Choline-binding protein	AF291696	M	Endo-β-N-acetylglucosaminidase (LytB) from S. pneumoniae (CAA 09078)	1	6/10	0/10
					2	7/10	0/10
Sp91	Choline-binding protein	AF291697	M	1,4-β-N-acetylmuramidase (LytC) from S. pneumoniae (CAA08765)	1	9/10	1/10
					2	10/10	1/10
Sp101	SPase II	AF291698	CW/M	Unknown protein from Bacillus anthracis (AAF13613)	1	7/10	0/10
				Unknown protein from Bacillus anthracis (AAF13613)	2	9/10	2/10
Sp128	Cell wall anchor	AF291699	CW/M	Cell wall-associated serine proteinase from S. pneumoniae (AAD48399)	1	9/10	0/10
					2	6/7	0/10
Sp130	Cell wall anchor	AF291699	CW/M	Cell wall-associated serine proteinase from S. pneumoniae (AAD48399)	1	9/10	0/10
					2	10/10	0/10
PspA	Control		CW/M		1	10/10	3/10
					2	10/10	0/10

CW, cell wall fraction; M, membrane fraction.

Homology searches were performed by comparing amino acid sequences against the nonredundant protein database using the BLAST algorithm at the National Center for Biotechnology Information. The most homologous amino acid sequence and its accession number are shown here.

The number of survivors compared to that for the sham-immunized control was found to be statistically significant (P < 0.05) for all groups by using a two-sample log rank test.