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. 2022 Dec 19;13:1078741. doi: 10.3389/fimmu.2022.1078741

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Copyright © 2022 Atmeh, Gay, Levasseur, La Scola, Olive, Mezouar, Gorvel and Mege

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MDM infection with SARS-CoV-2 variants. MDM were infected with SARS-CoV-2 variants including Wuhan (China), α-B.1.1.7 (United Kingdom, UK), β-B.1.351 (South Africa, SA), γ-P.1 (Brazil), δ-B.1.617 (India) and B.1.1.529 (Omicron) (0.1 MOI) for 6, 24, 48 and 72 hours. (A) SARS-CoV-2 was quantified in cells as Ct values by RT-PCR. (B) Cell viability was tested at 24, 48 and 72 hours post-infection. (C) SARS-CoV-2 replication was quantified by RT-PCR in cell supernatants and expressed as Ct values. Data values represent the mean ± SD from 4 healthy donors whose experiments were carried out in triplicate. Statistical analysis was performed with two-way ANOVA and Tukey’s multiple comparison test. ^*p ≤ 0.05, ^**p ≤0.01 and ^***p ≤ 0.001.