FIGURE 1.

(A) A scheme of the HAC/dGFP assay. The assay is based on the use of the HAC/dGFP (the alphoid^tetO-HAC containing the dGFP transgene). Cells with the HAC display green fluorescence, while cells that lack it do not. It is expected that untreated cells (“a negative control”) should show uniform green fluorescence. (B) A cell population, that has lost HAC upon treatment with the CIN-inducing drug (“a prospective compound”), loses GFP fluorescence. For the positive control, we used Taxol, a CIN-inducing microtubule destabilizing agent. The actual number and percentage of cells with the HAC/dGFP are measured by a fluorescence microplate reader and flow cytometry.