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. 2022 Dec 19;13:1081506. doi: 10.3389/fpls.2022.1081506

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Copyright © 2022 Tek, Calis, Fidan, Shah, Celik and Wani

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Combined DAB and Trypan blue staining to determine ROS accumulation on leaves of inoculated WT and mutant plants at 10 dpi. Although susceptible WT-ADR27 had highest P. xanthi germination rate, ROS accumulation (black arrow) was found more frequently on the leaves of these plant as compared 4-11 and 4-14. ROS accumulation was also found in 4-11 and 4-14 which are CsaMLO8/11^del and CsaMLO1 ^A137N/8/11^del mutants. However, ROS were accumulated in the P. xanthii mycelial growth site was detected in 4-11 and 4-14. Also, non-germinated spores (red arrow) were detected on mutant leaves on the contrary to WT.