TABLE 1.

Cytokine production by spleen and mesenteric lymph node cells from mice immunized intranasally with SAG1 protein plus toxin^a

Group	Amt released (pg/ml)
	IFN-γ		IL-2
	Alone	+ TLA	Alone	+ TLA
LTR72 + SAG1	23	1,414	20	361
LTK63 + SAG1	24	34	<15	<15
LT + SAG1	26	2,532	<15	163
CT + SAG1	<8	831	<15	141
SAG1	<8	<8	<15	16
LTR72	31	36	26	24
LTK63	29	219	18	30
LT	138	338	<15	<15
CT	119	387	34	35
Untreated	28	37	24	26
LTR72 + SAG1	75	198	<15	31
LTK63 + SAG1	35	209	<15	<15
LT + SAG1	<15	<15	<15	25
CT + SAG1	22	344	<15	39
SAG1	69	116	<15	<15
LTR72	100	235	16	<15
LTK63	104	235	<15	<15
LT	<15	<15	<15	<15
CT	40	180	<15	<15
Untreated	131	318	<15	<15

^aMice were primed by two intranasal immunizations and rested for 15 days before being killed. Single-cell suspensions were prepared from spleens and mesenteric lymph nodes and cultured in the presence or absence of T. gondii antigen (15 μg/ml). Amounts released were calculated with recombinant cytokine. Values for IL-2 and IFN-γ were taken from samples at 48 and 96 h, respectively. The results are representative of at least three experiments. Boldface indicates no significant concentration.