FIGURE 3.

Cinacalcet enhancement of slowly recovering VGSCs. (A) Representative traces from a protocol used to isolate and evaluate the voltage-dependence of slow inactivation. Measurements were taken by applying a 5 s depolarization to varying voltages (−140 to +20 mV in 10 mV increments) from a holding potential of −80 mV, returning to −120 mV for 100 ms to allow recovery from fast inactivation, and delivering a test pulse to −20 mV in control conditions (black) and following full inhibition by 5 µM cinacalcet (red). (B) Plot of average normalized peak VGSC currents elicited by test pulse following 5 s conditioning pulse and 100 ms recovery period in control (black; n = 6) and following full inhibition by cinacalcet (red; n = 6) fit to the Boltzmann equation. Dashed red line indicates average cinacalcet fit normalized to the average control maximum. (C) Plot of individual (black, open circles) and mean (red, filled circles) V_0.5 values. (D) Plot of individual (black, open circles) and mean (red, filled circles) apparent valence values.