Figure 2.

CLCa_E203A does not complement the biomass production deficiency of clca-2. Ws-2, clca-2, clca-2/35S:CLCa_E203A lines 8 and 3 and complemented line clca-2/35S:CLCa plants were grown hydroponically in 4.25 mM ${\text{NO}}_3^{-}$ under short-day conditions. After 4 weeks, photographs were taken (A) and after 6 weeks, shoot and root fresh weights and shoot/root ratio biomasses were measured (B). Data represent the means ± sem of three biological replicates (n = 3–6 plants per replicate). A Shapiro–Wilk normality test followed by a Welch’s t test was applied. Different letters indicate significant difference between genotypes (P < 0.05). Scale bar represents 1 cm.