Figure 5.

Dynamics of chromosomal behavior in WT and Osgsl5-2 anthers. Plastic-embedded anthers cross-sections stained with DAPI for chromosomes observation. A and I, Mitosis; B and J, Interphase; C and K, Leptotene; D and L, Zygotene; E and M, Pachytene; F and N, Diplotene; G and O, Diakinesis; H and P, Dyad. Bar = 20 µm. Note that Osgsl5-2 mutant anthers contained two types PMCs with respect to chromosome appearance (wl-PMC and ab-PMC, see the text). The insets in each panel are magnified views of nuclei enclosed with dashed squares, in which white and red squares indicate nuclei of wl-PMCs and ab-PMCs, respectively. The insets in (E) and (M) are further magnified and shown in E′, M′1, and M′2, as examples of WT PMC, wl-PMC, and ab-PMC, respectively.