Gene editing of CYCB1;1 and CDKB2;2 (cycb1;1-cr and cdkb2;2-cr, respectively) largely rescues the ABA-insensitive phenotype of abi4 with regard to primary root growth in response to ABA application. A, Primary root phenotype of Col-WT, abi4, cycb1;1-cr, cdkb2;2-cr, abi4/cycb1;1-cr, and abi4/cdkb2;2-cr of seedlings grown on 1/2 MS medium with or without exposure to ABA (10 μM). The 3-day-old seedlings on 1/2 MS were transferred to the medium with or without ABA at different concentrations, and the seedlings were harvested 2 days after transfer. Bar = 10 mm. B and C, The quantitative analysis of primary root length for (A) is shown. The values are means ± se. In one experiment at least three repeats, and in one repeat, >30 roots per genotype were used. Duncan’s test (P < 0.05) was employed to check statistically significant means. The same letters, no significant difference; different letters, significant difference.