Figure 7.

Expression pattern of DR5_promoter-GFP in the WT and abi4 mutant roots. A–D, Expression patterns of the DR5_promoter-GFP reporter gene in both genotypes grown on 1/2 MS (A and B), and ABA-containing media (C and D). E, Statistical analysis of GFP fluorescence levels for (A)–(D). The 4-day-old seedlings grown on 1/2 MS medium were sampled. Three-day-old seedlings on 1/2 MS medium were transferred to the ABA-containing medium (30 μM ABA), with the seedlings being harvested 2 days after transfer, for analysis. Bar = 50 mm. Five–ten images were examined per genotype, and at least three independent experiments were performed. The statistical significance of differences was evaluated by Student’s t test analysis. The values are means ± se. Asterisks indicate statistically significant differences from the control (CK), 0-h point (^*P < 0.05).