Figure 3.

Expression pattern, subcellular localization, and regulation analysis of SGC of BnaC2.MYB28. A, RT–qPCR analysis of BnaC2.MYB28 in NIL(ZY50) and NIL(G120). BnACTIN7 was used as a control. Error bars represent the sds from three independent RNA samples. B, Diagrams of the test constructs used in (C). C, Relative luciferase activity of promoters of BnaC2.MYB28^G120 and BnaC2.MYB28^ZY50 in Arabidopsis protoplasts. Each dot on the column represents an independent replicate. The activities of LUC were normalized as the ratio of LUC/REN activity. Data are shown as mean ± sd (n = 9). Error bars represent the sds. GUS analysis of BnaC2.MYB28^G120 (D) and BnaC2.MYB28^ZY50 (E) in transgenic wild-type Arabidopsis. Bars = 2 mm. F, Subcellular localization of BnaC2.MYB28 protein in Arabidopsis leaf protoplasts. The nuclear protein OsGHD7-fused CFP was used as a nuclear marker. Bars = 10 μm. G, SGC in G120, transgene-negative and transgene-positive T₁ lines (GZ−2, GZ−5, and GZ−6). Each dot on the column represents an individual plant. H, SGC in ZY50 and BnaC2.MYB28^ZY50 overexpression lines (ZOE−22 and ZOE−25) in the T₁ generation. Each dot on the column represents an individual plant. (I) SGC of the WT, transgene-negative and transgene-positive T₀ plants of the BnaC2.MYB28^G120-RNAi and BnaC2.MYB28^G120-OE transformants. Each dot on the column represents an individual plant. In (G), (H), and (I), data are shown as mean ± sd (n ≥ 6); error bars represent the sds; two-tailed Student’s t tests were used to generate the P-values. ***P < 0.001.