TABLE 2.
Activation markers on CD4 cells from mesenteric lymph nodes of control and C. parvum-infected micea
| Mouse examined | No. of mice | Infection status | % CD4 cells (mean ± 1 SD) staining for activation marker
|
|
|---|---|---|---|---|
| CD69 | CD154 | |||
| C57BL/6 | 6 | Uninfected control | 18 ± 5 | 5 ± 3 |
| C57BL/6 | 7 | C. parvum infected | 31 ± 3b | 12 ± 2b |
| CD154−/− C57BL/6 | 6 | Uninfected control | 14.6 ± 6.3 | <0.1 |
| CD154−/− C57BL/6 | 8 | C. parvum infected | 32 ± 2b | <0.1 |
| CD40−/− C57BL/6 | 5 | Uninfected control | 28 ± 4 | 7 ± 2 |
| CD40−/− C57BL/6 | 5 | C. parvum infected | 58 ± 3b | 15 ± 2b |
a
Mesenteric lymph nodes were recovered from the infected mice 6 days after infection. The cells were stained with p-conjugated CD4 antibody and fluorescein isothiocyanate antibodies to the activation markers, and 5,000 CD4 cells were examined. Results are the mean ± 1 SD of CD4 cells staining for the activation marker shown.
b
These results differ (P < 0.05 by Mann-Whitney U test) from the corresponding control.