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. 2022 Oct 12;191(1):382–399. doi: 10.1093/plphys/kiac472

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© The Author(s) 2022. Published by Oxford University Press on behalf of American Society of Plant Biologists.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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SL biosynthesis increased in Oszas2 mutants. A, Relative quantification of 4DO and 4-oxo-MeCLA in root tissue of Oszas2 mutants. B, Normalized expression value of SL biosynthetic genes in Oszas2 mutants. C, Relative quantification of canonical (4DO, Orobanchol) and noncanonical (4-oxo-MeCLA) SL in root exudate of Oszas2 mutants. D, Striga seed germination assay conducted with root exudate and tissue of Oszas2 mutants. For both root exudate and tissue bioassay, 1 μM of GR24 was used as control, which showed about 65% and 67% of Striga seed germination, respectively. Boxes in boxplots in (A) and (C) represent the median, first and third quartile. The minimum and maximum values are showed with the length of the whiskers. Dots represent the biological replicates. Values in (A–D) are means ± sd (n ≥ 4) and student’s t test was applied for the statistical analysis (*P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001).