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. 2022 Sep 22;191(1):542–557. doi: 10.1093/plphys/kiac447

Figure 4.

Figure 4

Hormone treatments reveal dysfunctional auxin-induced hyponasty in ABA mutants and reduced sensitivity to ABA in LRFR. A, Leaf elevation angle of Leaves 1 and 2 in Col-0 (black) and nced3nced5 mutant (blue) plants treated with mock solution (solid lines) or 10 μM IAA (dashed lines). At ZT3 on Day 15 (t=3) a 1-μL drop of the corresponding solution was applied to the leaf tip (adaxial side). Leaf elevation angles are mean values (n=22–30). B and C, Leaf elevation angle of Leaves 1 and 2 in Col-0 plants treated with mock (black), 1 μM ABA (B, red), and 10 μM ABA (C, green) solutions in high R/FR (solid) versus low R/FR (dashed) conditions. Leaf elevation angles are mean values (n=26–30). At ZT3 on Day 15 (t=3), the corresponding solution was sprayed on the entire leaves (adaxial side). Shade treatment started at t=3 by adding FR light to decrease the R/FR ratio. Col-0 plants analyzed in (B) and (C) are same. A–C, Plants were grown for 14 d in standard LD (16/8) conditions. Imaging started on Day 15 at ZT0 (t=0), plants were maintained in LD. Opaque bands around mean lines represent the 95% confidence interval of mean estimates. Vertical gray bars represent night periods.