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. 2022 Sep 10;191(1):515–527. doi: 10.1093/plphys/kiac423

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© The Author(s) 2022. Published by Oxford University Press on behalf of American Society of Plant Biologists.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Characterization of DEF8 at the reproductive stage. A, Spatio-temporal expression of DEF8 during reproductive growth. Histochemical assay of panicles from a transgenic plant harboring proDEF8::GUS at heading (left), filling (middle), and mature (right) stage. The part in the dashed box was enlarged to show staining of glume vasculature. The grain outlined in blue was shelled to brown rice. B, Cross-section of glume at heading stage. P, phloem; X, xylem. Bar = 10 µm. C, Imaging the cross-sectioned glume of transgenic plants harboring the construct proDEF8::_fDEF8-mRFP/pCAMBIA1300. Bar = 10 µm.