Figure 2.

Interaction between amino (N)-terminal halves of VvWRKY8 and VvMYB30. A, Y2H assay. VvMYB30-N-AD/BD, VvMYB30-C-AD/BD, AD/VvWRKY8-N-BD, and AD/VvWRKY8-C-BD were used as negative controls. B, Pull-down assay. VvWRKY8-N and VvWRKY8-C were fused with SUMO and HIS tags, and the molecular weights were almost 18 and 23 kD, respectively. VvMYB30-N and VvMYB30-C were fused with GST tag, and the molecular weight was almost 40 kD. VvMYB30 and GST were detected by GST tag monoclonal antibody, VvWRKY8 was detected by HIS tag monoclonal antibody. VvMYB30-N, N-terminal of VvMYB30; VvMYB30-C, C-terminal of VvMYB30; VvWRKY8-N, N-terminal of VvWRKY8; and VvWRKY8-C, C-terminal of VvWRKY8.