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. 2022 Oct 18;35(1):552–573. doi: 10.1093/plcell/koac308

Figure 4.

Figure 4

VvMYB30 overexpression and knock-down in grapevine leaves and berry skin alter STS15/21 and STSs expression and Res content. The vectors were transiently transformed into leaves and berry skin to increase or reduce expression level of MYB30. For MYB30 overexpression, full-length coding sequence of VvMYB30 was inserted into pSAK277 vector with MYB30 promoter cloned from V. amurensis (A). The vectors were transiently transformed into V. amurensis leaves and “Beixi” (V. vinifera “Muscat Hamburg” × V. amurensis) berry skin. Relative expression level of MYB30 and its target gene STS15/21 in V. amurensis leaves (B) and “Beixi” berry skin (C) infiltrated with MYB30 were measured by RT-qPCR. The contents of trans/cis-Pd and trans/cis-Res in leaves (D) and berry skin (E) infiltrated with MYB30 were measured by HPLC. For knocking down the expression of MYB30, a 250-bp fragment from VvMYB30 coding sequence was inserted into pFGC5941 vector in forward and reverse (F). The vectors were transiently transformed into V. amurensis leaves and “Kyoho” (V. labrusca × V. vinifera) berry skin. Relative expression level of MYB30 and its target gene STS15/21 in leaves (G) and berry skin (H) were measured by RT-qPCR. Res contents in leaves (I) and berry skin (J) were measured by HPLC. Data are means ± se of three biological replicates. Statistical significance was calculated using Student’s t test (*P < 0.05). NS, no significant difference.