Figure 1. Rab27a and Rab27b knockdown suppressed exosome secretion in gastric cancer cells. (A) RT-qPCR of Rab27a and Rab27b in 58As9 cells transfected with Rab27a or Rab27b shRNAs. (B) Immunoblotting of Rab27a and Rab27b in 58As9 cells transfected with Rab27a or Rab27b shRNAs. (C) Transmission electron microscopy image of exosomes obtained from the cell supernatants. (D) Immunoblotting of CD63 in the culture supernatant from 58As9 cells transfected with Rab27a or Rab27b shRNAs. shCtrl: shControl 58As9 cells; shRab27a: Rab27a shRNA-transfected 58As9 cells; shRab27b: Rab27b shRNA-transfected 58As9 cells.
