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. 2022 Aug 29;40(12):1149–1161. doi: 10.1093/stmcls/sxac060

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© The Author(s) 2022. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Isolation and characterization of keratinocyte subpopulations. (A) Keratinocytes were isolated based on their ability to adhere to type IV collagen. Four subpopulations were obtained: (1) KSC that adheres to the collagen in 5ʹ; (2) ETA cell that adheres in 15ʹ; (3) LTA cell that adheres overnight; (4) PM cell that does not adhere after 1 night. For comparison, KSC were isolated after 20ʹ. The relative β1-integrin expression of each subpopulation is shown by WB analysis. (B) Freshly isolated KSC 20ʹ, KSC 5ʹ, ETA, LTA, and PM cells were plated on the 3T3 feeder layer and CFE (C) and colony areas (D) were determined as described in Material & Methods section. Data are represented as mean ± SEM. Ordinary one-way ANOVA followed by Tukey’s multiple comparisons are represented. Comparisons with KSC5ʹ, unless differently indicated, are shown. ns: P > .05; * .01 < P < .05; ** P < .01; ***P < .001; ****P < .001.