Figure 5.

Generation of skin equivalents from LTA cells overexpressing CD271. (A) Confirmation of CD271 overexpression in protein extracts from mock or CD271 infected LTA cells by immunoblotting. (B) Skin equivalents derived from ETA, CD271 overexpressing LTA, and mock LTA cells were paraffin-embedded at 12 days from cell seeding, and sections were stained with H&E (B). Bar = 100 μm. Epidermal thickness was measured by Fiji-ImageJ software. (C) Sections of skin equivalents obtained from ETA, CD271 overexpressing LTA, and mock LTA cells, were stained with anti- Ki67 antibodies by IHC, anti-Survivin, anti-K15, and anti-Involucrin antibodies by IF. Bar = 100 μm. The number of positive cells or stained areas was evaluated by image pixel count using Fiji-ImageJ. Data are represented as mean ± SEM. Ordinary one-way ANOVA followed by Tukey’s multiple comparisons test were performed, and comparisons are indicated. ns: P > .05; *.01 < P < .05; ** P < .01; ***P < .001; ****P < .0001.