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Characterization of neural progenitors. (A) Expression of NNPs markers and PNPs markers projected onto the t-SNE map. (B) Immunofluorescence staining for 2 major neural progenitors (NNPs (SOX2+/Ki67−), and PNPs (SOX2+/Ki67+)). White arrows indicate NNPs, and yellow arrows indicate PNPs. Scale bar = 50 µm. (C) t-SNE projections of NNPs, which are further divided into 2 sub-clusters (NNPs-C1 and NNPs-C2). (D) Heatmap of differentially expressed genes between NNPs-C1 and NNPs-C2.
