(A) Schematic illustration of the interleaved simultaneous FUS + tactile and tactile stimulus presentation paradigm. Only one FUS intensity was presented in each fMRI run. (B) Time courses of % BOLD signal changes at the FUS target area 3a/3b, and five off-target regions of area 1/2, S2, insula, MCC, auditory cortex, and thalamus VPL and MD nuclei. Light orange and blue backgrounds indicate the 30 s duration of stimulation. (C) Bar plots of peak BOLD signal changes during tactile and FUS stimulation at three intensities shown for the target (light orange section) and off-target ROIs (light blue section). *p < 0.05, **p < 0.01, ***p < 0.001, ***p < 0.0001, where the analysis comprised a one-way ANOVA test followed by Tukey's test.