FIGURE 3.

Effects of avocado oil on NAFLD development. The images are representative of four independent hematoxylin and eosin histological examinations of livers dissected from different animals. Black arrows indicate the presence of steatosis; dotted black arrows indicate the presence of inflammatory infiltrates; circles indicate the presence of Mallory’s hyaline. The images were taken at ×10 magnification, while the images in the inserts were taken at ×20 magnification (A). Scoring of the percentage of steatosis and hypertrophy. The dotted lines delimit the percentage of steatosis or hypertrophy corresponding to different categories according to Liang et al. (2014): 0 (<5%), 1 (5%–33%), 2 (34%–66%) and 3 (>66%) (B). Scoring of inflammation: normal (<0.5), slight (0.5–1.0), moderate (1.0–2.0), severe (>2.0). These categories are delimited by dotted lines (C), according to the criteria by Liang et al. (2014). Data are expressed as the mean ± standard error of n = 3. ^* p < 0.05 vs. CTRL; ^# p < 0.05 vs. HF6; ^§ p < 0.05 vs. HF6+AVO + D; ^¶ p < 0.05 vs. HF12 (ANOVA, Holm-Šídák’s post hoc test).