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. 2001 Mar;69(3):1687–1696. doi: 10.1128/IAI.69.3.1687-1696.2001

FIG. 6.

FIG. 6

(A) Polymorphisms in the 3′ end of hemO and the intergenic space between this position and the exl3 start codon are characteristic of the exl3 alleles in N. meningitidis. Alignment of the nucleotide sequences from position 663 of hemO (position 1 in this figure) to position 288 of exl3 in 10 meningococcal isolates and N. lactamica is shown. The numbering of the polymorphic sites within the sequences is shown in vertical format above the sequence for N. lactamica exl3L (accession number AF319537). In this scheme, numbers 29 to 31 correspond to the stop codon of hemO and positions 164 to 166 correspond to the exl3 start codon. Nucleotide positions that are identical to those in N. lactamica exl3L are shown as dots, and dashes indicate spaces. Meningococcal isolates (ETs): B431 (375), 6083 (916), B472 (384), M0022 (504), M1978 (525), M1762 (509), Z5005 (723), M2436 (546), M2505 (548), and M2743 (800). (B) Polymorphisms in the first 650 bp of exl3L from N. meningitidis strain B431 (ET375) and N. lactamica exl3L. Identical nucleotides (dots) and spaces (dashes) are marked according to the reference sequence of exl3 from N. meningitidis strain 6083 (ET916). The polymorphic sites are numbered from positions 1 to 3 corresponding to the ATG start codon of exl3. Asterisks mark polymorphisms not shared between the exl3L cassettes in N. meningitidis strain B431 and N. lactamica. (C) Alignment of the complete exl3 genes from N. meningitidis strain 6083 (ET916) and N. meningitidis strain Z5035 (ET747). This alignment revealed polymorphisms in the nucleotide sequence that corresponds to the C-terminal domain of the translated protein. In this numbering scheme, positions 1 to 3 correspond to the ATG start codon of exl3 (see above for details).