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. 2021 Jun 4;12(3):151–160. doi: 10.1007/s42485-021-00066-x

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© The Author(s) 2021, corrected publication 2022

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Fig. 4 — Identifications of spliced peptides. A, B Bar graph of the total number of identified MHC-bound peptides and spliced peptides in A375 and Loucy cell lines. C, D Scatter plots of the predicted hydrophobicity index (HI) calculated using SSRCalc and observed retention times (RT) of the spliced peptides. As the peptides are separated based on their hydrophobicity on reverse-phase resin-based separation columns, a positive correlation of HI with peptide retention time indicates the reliability of the peptide sequence assignment