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. 2021 Jun 4;12(3):151–160. doi: 10.1007/s42485-021-00066-x

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© The Author(s) 2021, corrected publication 2022

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Fig. 5 — Validation of the identified peptides using synthetic peptide. Synthetic peptides were synthesized and analyzed by LC–MS/MS analysis. Peptides were validated by overlaying the experimental MS/MS spectrum with the synthetic MS/MS spectrum. Representative MS/MS spectrum was shown for A SEASPSREAI (phosphorylated peptide), B FVYNITTNK (deamidated peptide) and C, D spliced peptides (SAAERLLAF, FLFQDFLRQA)