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. 2022 Oct 1;114(1):174–186. doi: 10.1111/cas.15587

FIGURE 5.

FIGURE 5

CXC chemokine ligand 14 (CXCL14) regulates the protumorigenic effects through insulin‐like growth factor‐1 receptor (IGF‐1R) signaling. (A) Coimmunoprecipitation of CXCL14 with IGF‐1R in GBM8401 cells. (B) Immunoblotting of IGF‐1R pulled down from CXCL14‐His tagged in GBM8401 cells, using Nickel magnetic beads. (C,D) Western blot analysis and quantification for detecting IGF‐1R, AKT, and ERK phosphorylation in GBM8401 and GBM04T cells treated with CXCL14 (100 ng/mL) with or without the IGF‐1R inhibitor (IMC‐A12, 100 nM) for 15 min. Error bars, SD within triplicate experiments. *p < 0.05, *p < 0.01, compared with cells without CXCL14 and IMC‐A12. (E–H) Cell viability (E), migration (F), invasion (G), and neurosphere formation rates (H) of GBM8401 and GBM04T cells treated with CXCL14 (400 ng/ml) with or without the IGF‐1R inhibitor (IMC‐A12, 100 nM) in culture medium. (I,J) Representative micrographs (I) and average diameter (J) of neurospheres developed from 10 day cultures. Error bars, SD within triplicate experiments. ***p < 0.001, compared with cells without CXCL14 and IMC‐A12. #### p < 0.0001, compared with the cells with CXCL14 alone