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. 2023 Jan 2;13:28. doi: 10.1038/s41598-022-24424-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Ovariectomy of female Wistar rats. (A) Positioning of the animal in ventral decubitus. (B) Shaving of the lumbar region. (C) Longitudinal 10-mm surgical incision on the left side (SI). (D) Exploration of the left ovary (LO). (E) Contralateral posterior incision and exploration of the right ovary (RO). (F) Identification of the ovary (O), uterine tube (UT), and uterine horn (UH). (G) Dissection of the ovary (O). (H) Repositioning of the anatomical structures. (I) Suture of muscles and skin, respectively.