Table 1.

Primers used for PCR generation of PHB1-GFPN1 deletion constructs.

Deletion construct	Primers, first PCR amplification		Primers, second PCR amplification
	Pair	(Complementary sequence underlined)
PHB1-GFPΔ10–24	1	Sense: Primer 1 Antisense: 5′-CACATTATATAAGGCAGAGCCAATGGACTCAAA-3′	Sense: Primer 1 Antisense: Primer 2
	2	Sense: 5′-TCTGCCTTATATAATGTGGATGCTGGGCACAGAGCT-3′ Antisense: Primer 2
PHB1-GFPΔ24–53	1	Sense: Primer 1 Antisense: 5′-CGGGATGAGAAAATGAGTGTTCACCACGCCTCCTGC-3′	Sense: Primer 1 Antisense: Primer 2
	2	Sense: 5′-ACTCATTTTCTCATCCCGTGGGTACAGAAACCAAT -3′ Antisense: Primer 2
PHB1-GFPΔ54–123	1	Sense: Primer 1 Antisense: 5′-CCACGGGATGAGAAAATGAGTCCCTTCCCCTACCAC-3′	Sense: Primer 1 Antisense: Primer 2
	2	Sense: 5′-CATTTTCTCATCCCGTGGCGCATCTTCACCAGCATCGGA-3′ Antisense: Primer 2
PHB1-GFPΔ156–190	1	Sense: Primer 1 Antisense: 5′-GGCCCTCTCTGCTTCCTGCTCTGTAAGGTCGTCGCT-3′	Sense: Primer 1 Antisense: Primer 2
	2	Sense: 5′-CAGGAAGCAGAGAGGGCCAGATTTGTGGTGGAAAAG-3′ Antisense: Primer 2
PHB1-GFPΔ190–236	1	Sense: Primer 1 Antisense: 5′-TTCCAGCTTGCGCAGCTCCTGAGCCACCTGTTTGGC-3′	Sense: Primer 1 Antisense: Primer 2
	2	Sense: 5′-GAGCTGCGCAAGCTGGAAGCTGCAGAGGACATCGCG-3′ Antisense: Primer 2
PHB1-GFPΔ236–261	1	Sense: Primer 1 Antisense: 5′-GATCAGGCCATCCCCTGCAGTGGCCAGTGAGTTGGC-3′	Sense: Primer 1 Antisense: Primer 2
	2	Sense: 5′-GCAGGGGATGGCCTGATCGCGGGGCAGTCCGTGCTC-3′ Antisense: Primer 2

	Primer 1 (sense)	Primer 2 (antisense)
PHB1 primers	5′-CGCGCCAAGCTTATGGCTGCCAAAGTG-3′ HindIII site underlined	5′-AATTGGATCCCCTCCCTGGGGCAGCTGGA-3′ BamHI site underlined