Table 2.
The result of the species specificity test using real-time PCR primer sets
| NO | Family | Species | Artemisia capillaris | Artemisia iwayomogi | ||||
|---|---|---|---|---|---|---|---|---|
| ITS | accD | rpoB | matK | rpoB | ycf1 | |||
| Cut-off cycles | 26.14 | 28.61 | 28.35 | 30.46 | 27.08 | 28.51 | ||
| 1 | Compositae | Artemisia capillaris | ++a | ++ | ++ | − | − | − |
| 2 | Artemisia iwayomogi | −b | − | − | ++ | ++ | ++ | |
| 3 | Dendranthema indicum | − | − | − | − | − | − | |
| 4 | Cirsium japonicum | − | − | − | − | − | − | |
| 5 | Xanthium strumarium | − | − | − | − | − | − | |
| 6 | Taraxacum platycarpum | − | − | − | +c | − | − | |
| 7 | Carthamus tinctorius | − | − | − | − | − | − | |
| 8 | Dendranthema zawadskii | − | − | − | + | − | − | |
| 9 | Aster tataricus | − | − | − | − | − | − | |
| 10 | Artemisia annua | − | − | − | − | − | − | |
| 11 | Kalimeris yomena | − | − | − | + | − | − | |
| 12 | Ambrosia artemisiifolia | − | − | − | − | − | − | |
| 13 | Liliaceae | Veratrum maackii | − | − | − | − | − | − |
| 14 | Hemerocallis fulva | − | − | − | − | − | − | |
| 15 | Zingiberaceae | Curcuma longa | − | − | − | − | − | − |
| 16 | Graminae | Zea mays | − | − | − | − | − | − |
a++ Means that was amplified before cut-off Ct cycles
b− Means that was not amplified before 40 cycles
c+ Means that was amplified between more than cut-off Ct cycles and less than 40 cycles