Table 3.
Evaluation of slope, R2, and efficiency obtained by real-time PCR system
| DNA standard curve | Dry-treated binary mixture standard curve | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Target species | Primer | Y (Slope) | R2 | Efficiency (%) | Target species | Primer | Y (Slope) | R2 | Efficiency (%) | |
| A. capillaris | AC_ITS | -3.54 | 1 | 91.46 | A. capillaris | AC_ITS | -3.45 | 0.98 | 94.89 | |
| AC_accD | -3.43 | 1 | 95.61 | AC_accD | -3.25 | 0.98 | 103.02 | |||
| AC_rpoB | -3.36 | 1 | 98.29 | AC_rpoB | -3.22 | 0.98 | 104.37 | |||
| A. iwayomogi | AI_matK | -3.54 | 1 | 91.93 | A. iwayomogi | AI_matK | -3.16 | 0.99 | 107.11 | |
| AI_ycf1 | -3.5 | 0.99 | 92.91 | AI_ycf1 | -3.21 | 0.99 | 104.58 | |||
| AI_rpoB | -3.57 | 0.99 | 90.28 | AI_rpoB | -3.17 | 1 | 106.51 | |||
| Heat-treated binary mixture standard curve | Autoclave-treated binary mixture standard curve | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Target species | Primer | Y (Slope) | R2 | Efficiency (%) | Target species | Primer | Y (Slope) | R2 | Efficiency (%) | |
| A. capillaris | AC_ITS | -3.21 | 0.99 | 104.75 | A. capillaris | AC_ITS | -3.29 | 0.99 | 101.39 | |
| AC_accD | -3.29 | 0.99 | 101.45 | AC_accD | -3.21 | 0.99 | 106.61 | |||
| AC_rpoB | -3.31 | 0.99 | 100.27 | AC_rpoB | -3.18 | 0.99 | 106.19 | |||
| A. iwayomogi | AI_matK | -3.17 | 0.99 | 106.76 | A. iwayomogi | AI_matK | -3.15 | 0.99 | 107.74 | |
| AI_ycf1 | -3.13 | 0.99 | 108.3 | AI_ycf1 | -3.14 | 0.99 | 108.15 | |||
| AI_rpoB | -3.14 | 0.99 | 108.15 | AI_rpoB | -3.12 | 0.99 | 108.9 | |||
Set threshold cycle (Ct) values obtained from three condition (dried, heated, and autoclaved) using the designed primer sets with reference binary mixtures