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. 2022 Sep 21;32(1):59–69. doi: 10.1007/s10068-022-01166-0

Table 4.

Results of the blind mixture (total mass of 150 mg) test for evaluating the reliability of the developed primer

A
Primer sets A. capillaris and A. iwayomogi blind test
Sample number 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20
18 s rRNA primer 13.76 ± 0.146 13.641 ± 0.317 13.679 ± 0.280 13.878 ± 0.144 13.582 ± 0.190 13.041 ± 0.235 13.531 ± 0.429 13.225 ± 0.213 12.99 ± 0.000 13.102 ± 0.094 13.621 ± 0.125 13.507 ± 0.225 13.438 ± 0.285 13.667 ± 0.142 13.458 ± 0.148 13.618 ± 0.122 13.671 ± 0.115 13.438 ± 0.113 13.087 ± 0.123 13.099 ± 0.106
A. capillaris
 ITS 26.14 cyclesa 19.785 ± 0.109 21.841 ± 0.093 22.146 ± 0.261 22.216 ± 0.212 19.876 ± 0.109 21.87 ± 0.166 22.34 ± 0.173 21.277 ± 0.127 33.232 ± 1.478 20.889 ± 0.081 22.015 ± 0.070 24.702 ± 0.162 21.296 ± 0.052 32.707 ± 0.435 21.858 ± 0.154 21.055 ± 0.140 22.166 ± 0.191 23.188 ± 0.254 31.704 ± 0.354 23.459 ± 0.238
 accD 28.61 cycles 22.924 ± 0.063 25.009 ± 0.035 25.859 ± 0.169 25.861 ± 0.111 23.466 ± 0.156 25.165 ± 0.268 25.72 ± 0.008 24.729 ± 0.240 34.872 ± 0.820 23.964 ± 0.142 25.521 ± 0.153 27.919 ± 0.068 24.766 ± 0.064 34.71 ± 0.301 24.795 ± 0.209 24.383 ± 0.096 25.716 ± 0.115 26.131 ± 0.209 33.891 ± 0.960 26.397 ± 0.085
 rpoB 28.35 cycles 23.153 ± 0.091 25.167 ± 0.029 25.886 ± 0.066 25.931 ± 0.062 23.721 ± 0.026 25.182 ± 0.130 25.8 ± 0.002 24.919 ± 0.048 35.344 ND 24.348 ± 0.185 25.8 ± 0.045 28.107 ± 0.068 24.832 ± 0.028 37.466 ± 2.853 24.951 ± 0.193 23.909 ± 0.235 25.879 ± 0.130 26.076 ± 0.160 33.212 ± 0.762 26.556 ± 0.167
A. iwayomogi
 matK 30.46 cycles 21.23 ± 0.022 21.144 ± 0.085 21.023 ± 0.022 21.363 ± 0.099 20.887 ± 0.150 20.731 ± 0.084 20.91 ± 0.051 20.565 ± 0.093 21.095 ± 0.067 20.558 ± 0.089 21.018 ± 0.032 20.985 ± 0.021 20.816 ± 0.139 20.985 ± 0.035 20.952 ± 0.046 20.946 ± 0.101 20.891 ± 0.128 20.812 ± 0.094 20.659 ± 0.021 20.589 ± 0.093
 ycf1 27.08 cycles 23.084 ± 0.119 23.108 ± 0.037 23.334 ± 0.194 23.239 ± 0.177 23.269 ± 0.158 23.019 ± 0.290 22.979 ± 0.192 22.919 ± 0.079 23.196 ± 0.183 22.895 ± 0.149 23.137 ± 0.121 23.07 ± 0.111 23.16 ± 0.252 23.286 ± 0.201 23.137 ± 0.355 23.01 ± 0.147 22.883 ± 0.116 22.83 ± 0.184 22.793 ± 0.154 22.795 ± 0.193
 rpoB 28.51 cycles 22.135 ± 0.105 22.211 ± 0.039 22.296 ± 0.058 21.942 ± 0.068 21.875 ± 0.131 21.727 ± 0.007 21.969 ± 0.024 21.841 ± 0.001 21.993 ± 0.012 21.783 ± 0.074 22.129 ± 0.157 22.026 ± 0.093 22.165 ± 0.052 22.082 ± 0.033 21.98 ± 0.003 21.877 ± 0.062 21.893 ± 0.112 21.732 ± 0.015 21.733 ± 0.087 21.704 ± 0.064
Test results O O O O O O O O O O O O O O O O O O O O
B
Primer sets A. capillaris and A. iwayomogi blind test
Sample number 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40
18 s rRNA primer 13.392 ± 0.091 13.18 ± 0.112 13.1 ± 0.077 13.094 ± 0.188 13.176 ± 0.096 12.915 ± 0.079 13.112 ± 0.064 12.904 ± 0.012 12.847 ± 0.115 13.039 ± 0.165 13.19 ± 0.101 13.37 ± 0.100 13.059 ± 0.140 13.343 ± 0.091 13.054 ± 0.053 13.22 ± 0.128 13.073 ± 0.205 13.005 ± 0.100 13.041 ± 0.171 12.81 ± 0.106
A. capillaris
 ITS 26.14 cyclesa 15.604 ± 0.377 14.895 ± 0.095 14.88 ± 0.142 15.596 ± 0.397 15.26 ± 0.049 14.856 ± 0.117 15.126 ± 0.091 14.883 ± 0.119 15.214 ± 0.094 14.929 ± 0.039 14.797 ± 0.114 15.582 ± 0.114 14.805 ± 0.093 15.287 ± 0.203 14.973 ± 0.152 15.194 ± 0.101 14.921 ± 0.146 15.306 ± 0.048 15.477 ± 0.006 14.98 ± 0.072
 accD 28.61 cycles 18.895 ± 0.141 18.473 ± 0.057 18.402 ± 0.133 18.294 ± 0.081 18.371 ± 0.053 18.259 ± 0.034 18.287 ± 0.072 18.378 ± 0.037 18.175 ± 0.075 18.172 ± 0.013 18.206 ± 0.248 18.362 ± 0.111 18.223 ± 0.050 18.356 ± 0.108 18.188 ± 0.188 18.229 ± 0.035 18.159 ± 0.051 17.993 ± 0.082 18.106 ± 0.008 17.899 ± 0.039
 rpoB 28.35 cycles 18.578 ± 0.050 17.994 ± 0.048 18.079 ± 0.034 17.997 ± 0.058 18.03 ± 0.027 18.026 ± 0.136 17.94 ± 0.026 17.955 ± 0.014 17.955 ± 0.009 17.974 ± 0.036 18.058 ± 0.019 18.26 ± 0.107 18.074 ± 0.030 18.192 ± 0.034 18.03 ± 0.050 18.09 ± 0.024 18.032 ± 0.052 17.949 ± 0.063 18.008 ± 0.112 17.888 ± 0.030
A. iwayomogi
 matK 30.46 cycles 22.863 ± 0.001 23.514 ± 0.061 24.936 ± 0.042 34.519 ± 1.008 23.772 ± 0.104 23.006 ± 0.151 26.165 ± 0.000 24.204 ± 0.083 26.98 ± 0.119 22.925 ± 0.011 22.945 ± 0.184 24.096 ± 0.031 24.448 ± 0.021 24.834 ± 0.085 26.111 ± 0.196 24.728 ± 0.171 34.742 ± 1.218 34.385 ± 0.581 24.827 ± 0.048 26.191 ± 0.261
 ycf1 27.08 cycles 24.776 ± 0.101 25.059 ± 0.051 26.09 ± 0.190 34.323 ± 0.190 25.74 ± 0.108 25.174 ± 0.153 27.614 ± 0.198 25.747 ± 0.110 28.195 ± 0.114 24.513 ± 0.288 26.148 ± 0.176 25.848 ± 0.103 26.122 ± 0.185 26.075 ± 0.122 27.339 ± 0.183 26.605 ± 0.172 35.054 ± 0.094 33.2 ± 0.186 26.237 ± 0.150 28.222 ± 0.179
 rpoB 28.51 cycles 24.237 ± 0.134 24.571 ± 0.080 25.817 ± 0.031 31.722 ± 0.020 24.955 ± 0.135 24.499 ± 0.117 26.966 ± 0.013 25.17 ± 0.194 27.929 ± 0.033 23.935 ± 0.044 25.701 ± 0.069 25.02 ± 0.062 25.148 ± 0.004 25.736 ± 0.037 26.795 ± 0.189 26.15 ± 0.006 32.065 ± 0.095 31.939 ± 0.055 25.68 ± 0.041 27.034 ± 0.009
Test results O O O O O O O O O O O O O O O O O O O O