Table 5.
Results of the real-time PCR assay using “Injin” commercial herbal medicines
| Primer sets | Labelled "InJin" commercial herbal medicines test | |||||||
|---|---|---|---|---|---|---|---|---|
| Dried leaves | ||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | |
| 18 s rRNA primer | 12.873 ± 0.098 | 14.301 ± 0.079 | 17.377 ± 0.052 | 15.872 ± 0.060 | 13.153 ± 0.040 | 13.026 ± 0.083 | 14.245 ± 0.445 | 13.785 ± 0.058 |
| A. capillaris | ||||||||
| ITS 26.14 cycles | 14.617 ± 0.114 | 30.918 ± 0.046 | 20.861 ± 0.117 | 19.415 ± 0.369 | 33.112 ± 0.106 | 34.693 ± 0.543 | 34.968 ± 0.762 | 33.457 ± 0.210 |
| accD 28.61 cycles | 17.951 ± 0.129 | 31.62 ± 0.546 | 23.537 ± 0.018 | 23.228 ± 0.014 | 34.823 ± 0.469 | 34.955 ± 0.493 | 35.009 ± 0.000 | 35.365 ± 0.112 |
| rpoB 28.35 cycles | 17.929 ± 0.091 | 31.837 ± 0.501 | 24.365 ± 0.107 | 23.41 ± 0.031 | 34.507 ± 0.349 | 33.909 ± 0.029 | 36.001 ± 3.352 | 33.78 ± 1.376 |
| A. iwayomogi | ||||||||
| matK 30.46 cycles | 35.033 ± 0.310 | 36.805 ± 0.270 | 33.993 ± 2.123 | 30.598 ± 0.408 | 21.801 ± 0.190 | 23.117 ± 0.239 | 20.756 ± 0.076 | 22.136 ± 0.156 |
| ycf1 27.08 cycles | 31.762 ± 0.392 | 37.016 ± 0.127 | 33.007 ± 0.145 | 30.993 ± 0.071 | 21.212 ± 0.002 | 20.826 ± 0.004 | 20.909 ± 0.068 | 19.129 ± 0.004 |
| rpoB 28.51 cycles | 34.458 ± 0.110 | 34.564 ± 0.473 | 34.504 ± 0.072 | 34.313 ± 0.102 | 22.491 ± 0.105 | 22.257 ± 0.043 | 22.098 ± 0.022 | 20.443 ± 0.143 |
| Primer sets | Labelled "InJin" commercial herbal medicines test | |||||||
|---|---|---|---|---|---|---|---|---|
| Dried leaves | ||||||||
| 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | |
| 18 s rRNA primer | 14.048 ± 0.035 | 14.048 ± 0.035 | 14.282 ± 0.038 | 14.925 ± 0.060 | 14.018 ± 0.054 | 14.819 ± 0.058 | 14.147 ± 0.148 | 13.716 ± 0.027 |
| A. capillaris | ||||||||
| ITS 26.14 cycles | 32.495 ± 0.396 | 34.046 ± 0.115 | 35.777 ± 1.692 | 35.231 ± 0.308 | 33.69 ± 0.268 | 35.724 ± 0.248 | 34.534 ± 0.772 | ND |
| accD 28.61 cycles | 34.917 ± 0.725 | 35.843 ± 0.482 | 37.244 ± 2.596 | 35.469 ± 0.161 | 36.521 ± 2.307 | 35.232 ± 0.506 | 36.867 ± 1.893 | 36.298 ± 0.835 |
| rpoB 28.35 cycles | 36.466 ± 2.339 | 35.364 ± 0.826 | 33.822 ± 1.796 | 35.011 ± 0.000 | 37.274 ± 2.579 | 38.643 ± 0.000 | 33.904 ± 0.031 | 28.231 ± 0.352 |
| A. iwayomogi | ||||||||
| matK 30.46 cycles | 23.674 ± 0.179 | 25.251 ± 0.729 | 21.098 ± 0.292 | 23.672 ± 0.244 | 22.967 ± 0.037 | 21.387 ± 0.295 | 22.043 ± 0.365 | 22.854 ± 0.049 |
| ycf1 27.08 cycles | 20.993 ± 0.044 | 21.472 ± 0.027 | 19.961 ± 0.029 | 20.742 ± 0.064 | 19.059 ± 0.081 | 19.122 ± 0.033 | 21.282 ± 0.044 | 19.984 ± 0.010 |
| rpoB 28.51 cycles | 22.083 ± 0.095 | 22.616 ± 0.059 | 21.263 ± 0.190 | 22.036 ± 0.161 | 20.747 ± 0.069 | 17.96 ± 0.034 | 22.362 ± 0.088 | 21.286 ± 0.057 |