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Outline of in utero ICV injection at E13.5
(A–F) The schematic shows key steps of the surgical procedure: (A) Anesthesia with isoflurane, (B) Abdomen hair removal, (C) Incision of skin and abdominal wall, (D) Expose embryos, and (E) Inject virus into the lateral ventricle.
(F) Injection site: Suture the abdominal cavity and skin followed by recovery and delivery.
Figure created with BioRender.com.
