REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit monoclonal anti-Superoxide dismutase 3 (1:1000)	Abcam	Cat#ab171738
Rabbit polyclonal anti-Albumin (1:1000)	Cell Signaling Technology	Cat#4929; RRID: AB_2225785
Rabbit monoclonal anti-Vinculin (1:1000)	Cell Signaling Technology	Cat#13901; RRID: AB_2728768
Anti-rabbit IgG, HRP-linked antibody (1:2000)	Cell Signaling Technology	Cat#7074; RRID: AB_2099233
Bacterial and virus strains
AAV2/5-CMV-hSOD3-hrGFP-hGH	Boston Children’s Hospital viral core, IDDRC	N/A
Chemicals, peptides, and recombinant proteins
Isoflurane	Piramal Critical Care	Cat#6679401710
Pivetal Alloxate (Meloxicam)	Patterson Veterinary	Cat#:07-893-7565
Optixcare eye lubricant	Patterson Veterinary	Cat#07-893-2779
1× PBS	GIBCO	Cat#10010023
10× HBSS	GIBCO	Cat#14180-020
Povidone-Iodine swabsticks	Fisher Scientific	Cat#06-669-82
Pierce BCA protein assay	Thermo Fisher Scientific	Cat#23227
RIPA lysis and extraction buffer	Thermo Fisher Scientific	Cat#89900
Halt protease and phosphatase inhibitor cocktail	Thermo Fisher Scientific	Cat#78400
NuPAGE 4%–12% Bis-Tris protein gels	Invitrogen	Cat#NP0336
NuPAGE LDS sample buffer	Invitrogen	Cat#NP0007
NuPAGE sample reducing agent	Invitrogen	Cat#NP0009
GE Healthcare Amersham ECL select	Fisher Scientific	Cat#45-000-999
Amersham Hybond P membranes, PVDF	Millipore Sigma	Cat#GE10600029
Critical commercial assays
Fast green	Sigma-Aldrich	Cat#F7252
Experimental models: Organisms/strains
Mouse: CD1 E13.5 pregnant dams	Charles River Laboratories	Cat#022
Recombinant DNA
pcDNA3.1-myc HisA (-) human SOD3	Ota et al.6	N/A
Other
Micropipette puller model PC-10	Narishige	Cat#PC-10
Microgrinder	Narishige	Cat#EG-45
Isoflurane anesthesia machine with induction chamber and nose cone	VETEQUIP	Cat#901806
Heating pad	Adroit Medical	Cat#HTP-1500
Water bath	VWR	Cat#WB02
LED gooseneck illuminator	LAXCO	Cat#AMPS-ILED-21
Glass bead sterilizer	Cellpoint Scientific	Cat#5-1450
Drummond calibrated micropipets	Fisher Scientific	Cat#21-176-2C
Nair hair removal cream	Nair	N/A
Disposable surgical drape	Fisher Scientific	Cat#NC0251373
Weigh scale	Parkland Scientific	Cat#KD-160
Reli silk non-absorbable sutures	VWR	Cat#89219-034
Adson forceps	Fine Science Tools	Cat#11006-12
Halsted-mosquito hemostats	Fine Science Tools	Cat#91308-12
Fine scissors	Fine Science Tools	Cat#14060-10
Nonwoven gauze sponges	Fisher Scientific	Cat#22-028-558
Protexis latex sterile surgical gloves	VWR	Cat#89233-804
Dropper bulb	Fisher Scientific	Cat#03-448-26
Aspirator tube assemblies for microcapillary pipettes	Millipore Sigma	Cat#A5177
Puritan sterile cotton-tipped applicator	VWR	Cat#10805-144
Needle insulin syringe	Fisher Scientific	Cat#22-004-270
Sterile transfer pipette	Sigma-Aldrich	Cat#Z350818
Absorbent underpads	VWR	Cat#56617-016
Pellet pestle cordless motor homogenizer	Fisher Scientific	Cat# 12-141-362