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. 2022 Dec 20;13:1017780. doi: 10.3389/fimmu.2022.1017780

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Copyright © 2022 Al-Saafeen, Al-Sbiei, Bashir, Mohamed, Masad, Fernandez-Cabezudo and al-Ramadi

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Treating MC38 tumor-bearing mice with Salmonella increases the antigen presentation potential of CD11b⁺ Ly6C^hi intratumoral myeloid cells. MC38 tumors were collected from control and Salmonella-treated mice on day 21 post tumor implantation and the percentages of different intratumoral immune cells were determined using flow cytometry. Representative dot plots and the combined results analyses for the percentages of CD11b⁺ (A, B), Ly6G⁺ (C, D), Ly6C^hi (C, E) intratumoral myeloid cells and MHC II-expressing cells (gated on CD11b⁺ Ly6C^hi cells) (F, G) are shown for each group. The expression of MHC II was evaluated using MFI (Median Fluorescence Intensity). (H). Each data point represents a single mouse, pooled from 3 independent experiments. Asterisks denote statistically significant differences from control group, **** (P ≤ 0.0001) and ns (no statistical significance, ≥ 0.05).