Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Dec 20;12:1049065. doi: 10.3389/fcimb.2022.1049065

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2022 Healer, Thompson, Mackwell, Browne, Seager, Ngo, Lowes, Silk, Pulido, King, Christen, Noe, Kotraiah, Masendycz, Rajagopalan, Lucas, Stanford, Soisson, Diggs, Miller, Youll, Wycherley, Draper and Cowman

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Strategy and antigens in the RCR complex used in single, double, and triple combinations to determine immunogenicity and ability to block P. falciparum growth. (A) Workflow of experiments to determine immunogenicity and development of growth inhibitory antibodies for the RCR complex. Adjuvants and antigen dose were determined prior to immunisation experiments using combinations of RH5.1, CyRPA and Ripr. (B) Schematic of recombinantly expressed proteins expressed with C-terminal affinity tags. RH5.1 (RH5) and CyRPA were expressed with a C-tag (EPEA) (purple) and Ripr was expressed with a StrepII tag (green) for purification. CyRPA and RH5 sequences were modified from the 3D7 wildtype sequence (at numbered positions) to remove potential N-linked glycosylation sites. RH5.1 and Ripr were expressed in S2 Drosophila cells, whereas CyRPA was expressed in mammalian HEK293 cells. (C) RH5.1, CyRPA and Ripr and RCR complex purified antigens analysed by SDS-PAGE and Coomassie staining to visualize the bands.