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. 2022 Dec 20;12:1049065. doi: 10.3389/fcimb.2022.1049065

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Copyright © 2022 Healer, Thompson, Mackwell, Browne, Seager, Ngo, Lowes, Silk, Pulido, King, Christen, Noe, Kotraiah, Masendycz, Rajagopalan, Lucas, Stanford, Soisson, Diggs, Miller, Youll, Wycherley, Draper and Cowman

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Depletion of specific antibodies to determine effect on inhibition of P. falciparum growth. IgG pools were made from the RCR equimolar antigen cohort IgG (A) and RCR mass cohort IgG (B) and incubated with antigens to deplete specific antibodies. Black bars - before depletion with antigen and others after depletion with specific antigen as indicated. Open bars – single antigen treatment (RCR complex is counted here as a single antigen). Hatched bars - antigen combinations. (C) Data extracted from panels (A, B) showing that GIA of RCR equimolar IgG and RCR mass IgG following CyRPA-depletion are not significantly different from each other. (A–C) Histograms show mean ± SD of two independent experiments (each performed in triplicate wells). ns, not significant.