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. 2022 Dec 5;299(1):102770. doi: 10.1016/j.jbc.2022.102770

Figure 3.

Figure 3

G4-stabilizing drugs and FANCJ deficiency induce mitotic recombination at G4s.A, schematic drawing of the model to show fork stalling, fork breakage, and HR repair at G4 sites. B and C, U2OS [HR-EGFP (TPG4)] cells treated with PDS (50 μM, 72 h) (B) and indicated concentrations of CX-5461 (72 h) (C) or without (No), and mitotic recombination was determined by FACS analysis of EGFP-positive cells. D, U2OS [HR-EGFP (TPG4)] cells were depleted for FANCJ by shRNA using vector (Vec) as control, and mitotic recombination was determined by FACS analysis 4 or 8 days after infection of FANCJ shRNA lentiviruses. FANCJ depletion is shown by Western blot with KU70 as a loading control. In all experiments, error bars represent the SD of at least three independent experiments. EGFP, enhanced GFP; FACS, fluorescence activated cell sorting; G4, G-quadruplexes; HR, homologous recombination; PDS, pyridostatin.