Key resources	Source	Identifier
Bacterial or viral strains
Escherichia coli XL10-GOLD KanR ultracompetent cells	Agilent	200317
OverExpress C41(DE3) chemically competent cells	MilliporeSigma	CMC0017
Chemicals, peptides, and recombinant proteins
Deuterium oxide 99.9%	Sigma–Aldrich	151882-10X1ML
GTPγS tetralithium salt	Sigma–Aldrich	G8634
ATP	Sigma	A7699-1g
MgCl2 ∗ 6H2O	Bio Basic	7791-18-6
Recombinant DNA
WDR44 (Homo sapiens) in pDONR221	DNASU	hsCD00296048
Strep-His WDR44 (334–504)	This article	NH17
WDR44 (334–504) cleaved	This article	NH22
WDR44 (334–504) glutathione-S-transferase	This article	NH22
Strep-His WDR44 (334–389)	This article	NH63
Strep-His WDR44 (340–389)	This article	MJ234
Strep-His WDR44 (334–389) L354A	This article	MT12
Strep-His WDR44 (334–389) I359A	This article	MT15
Strep-His WDR44 (334–389) K360E	This article	MT14
Strep-His WDR44 (334–389) L370A	This article	MT11
WDR44 (334–389) S344D	This article	NH64
Rab11a	PMID: 33277614	MF19
Rab11a Q70L		JB88
Rab11a Q70L A75T	This article	MT16
Rab25	This article	MT17
Rab2a	PMID: 33277614	MJ31
Rab4b	PMID: 33277614	MJ30
Rab1	PMID: 33277614	MJ118
Rab5a	PMID: 33277614	DH1
Sgk3	PMID: 34181950	Sgk3
Software and algorithms
HDExaminer	Sierra Analytics	http://massspec.com/hdexaminer/
GraphPad Prism 7	GraphPad	https://www.graphpad.com/scientific-software/prism/
PyMOL	Schrödinger	https://pymol.org/
Adobe Illustrator 2022	Adobe	https://www.adobe.com/products/illustrator.html
Other
Octet HIS1K Biosensors	Sartorius	18-5120