Figure 2.

Oncoproteoform plot describing the manually validated KRAS4A (light blue) and KRAS4B proteoforms and the contexts in which they were identified. Each row represents all validated proteoforms that share the combinations of PTMs indicated with “X”s on the left. The number of unique proteoforms in each row is shown on the right. In the middle section, the presence or the absence of the proteoforms in the biological contexts (cell lines [light gray] and colorectal tumors) is displayed. The color of the box indicates the expressing allele of validated proteoforms: purple denotes WT form only, magenta is mutant form only, and green represents both WT and mutant forms. No fill indicates no proteoform was detected. The dark yellow row shows proteoforms bearing a C185∗ truncation, whereas light yellow columns in the left section highlight modifications, which involve the C-terminal residue C185. Dark blue bars on top indicate the total number of validated KRAS proteoforms in each context. PTM, post-translational modification.